| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Monoclonal |
| Clone ID: |
S4107RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BCKDK |
| Gene ID: |
10295 |
| Uniprot ID: |
BCKD_HUMAN |
| Immunogen Region: |
1-100 |
| Specificity: |
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BCKDK (O14874). |
| Immunogen Sequence: |
MILASVLRSGPGGGLPLRPL LGPALALRARSTSATDTHHV EMARERSKTVTSFYNQSAID AAAEKPSVRLTPTMMLYAGR SQDGSHLLKSARYLQQELPV |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Autophosphorylated. |
| Function | Serine/threonine-protein kinase component of macronutrients metabolism. Forms a functional kinase and phosphatase pair with PPM1K, serving as a metabolic regulatory node that coordinates branched-chain amino acids (BCAAs) with glucose and lipid metabolism via two distinct phosphoprotein targets: mitochondrial BCKDHA subunit of the branched-chain alpha-ketoacid dehydrogenase (BCKDH) complex and cytosolic ACLY, a lipogenic enzyme of Krebs cycle. Phosphorylates and inactivates mitochondrial BCKDH complex a multisubunit complex consisting of three multimeric components each involved in different steps of BCAA catabolism: E1 composed of BCKDHA and BCKDHB, E2 core composed of DBT monomers, and E3 composed of DLD monomers. Associates with the E2 component of BCKDH complex and phosphorylates BCKDHA on Ser-337, leading to conformational changes that interrupt substrate channeling between E1 and E2 and inactivates the BCKDH complex. Phosphorylates ACLY on Ser-455 in response to changes in cellular carbohydrate abundance such as occurs during fasting to feeding metabolic transition. Refeeding stimulates MLXIPL/ChREBP transcription factor, leading to increased BCKDK to PPM1K expression ratio, phosphorylation and activation of ACLY that ultimately results in the generation of malonyl-CoA and oxaloacetate immediate substrates of de novo lipogenesis and glucogenesis, respectively. Recognizes phosphosites having SxxE/D canonical motif. |
| Protein Name | Branched-Chain Alpha-Ketoacid Dehydrogenase KinaseBckdh KinaseBckdhkinBdk3-Methyl-2-Oxobutanoate Dehydrogenase Lipoamide Kinase - Mitochondrial |
| Database Links | Reactome: R-HSA-70895 |
| Cellular Localisation | Mitochondrion MatrixDetected In The Cytosolic Compartment Of Liver Cells |
| Alternative Antibody Names | Anti-Branched-Chain Alpha-Ketoacid Dehydrogenase Kinase antibodyAnti-Bckdh Kinase antibodyAnti-Bckdhkin antibodyAnti-Bdk antibodyAnti-3-Methyl-2-Oxobutanoate Dehydrogenase Lipoamide Kinase - Mitochondrial antibodyAnti-BCKDK antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
