• Western blot analysis of extracts of various cell lines, using BCKDHA antibody (STJ111848) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of NIH/3T3 cells using BCKDHA rabbit polyclonal antibody (STJ111848) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-BCKDHA antibody (276-445) (STJ111848)

SKU:
STJ111848

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BCKDHA (276-445) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCKDHA
Gene ID: 593
Uniprot ID: ODBA_HUMAN
Immunogen Region: 276-445
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 276-445 of human BCKDHA (NP_000700.1).
Immunogen Sequence: SEQYRGDGIAARGPGYGIMS IRVDGNDVFAVYNATKEARR RAVAENQPFLIEAMTYRIGH HSTSDDSSAYRSVDEVNYWD KQDHPISRLRHYLLSQGWWD EEQEKAWRKQSRRKVMEAFE QAERKPKPNPNLLFSDVYQE MPAQLRKQQESLARHLQTYG EHYPLDHFDK
Function Together with BCKDHB forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction.
Protein Name 2-Oxoisovalerate Dehydrogenase Subunit Alpha - Mitochondrial
Branched-Chain Alpha-Keto Acid Dehydrogenase E1 Component Alpha Chain
Bckde1a
Bckdh E1-Alpha
Database Links Reactome: R-HSA-389661
Reactome: R-HSA-70895
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-2-Oxoisovalerate Dehydrogenase Subunit Alpha - Mitochondrial antibody
Anti-Branched-Chain Alpha-Keto Acid Dehydrogenase E1 Component Alpha Chain antibody
Anti-Bckde1a antibody
Anti-Bckdh E1-Alpha antibody
Anti-BCKDHA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance