• Immunofluorescence analysis of PC-12 cells using BCAT1 Rabbit polyclonal antibody (STJ118779) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using BCAT1 Rabbit polyclonal antibody (STJ118779) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using BCAT1 Rabbit polyclonal antibody (STJ118779) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using BCAT1 Rabbit polyclonal antibody (STJ118779) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using BCAT1 Rabbit polyclonal antibody (STJ118779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BCAT1 antibody (20-190) (STJ118779)

SKU:
STJ118779

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCAT1
Gene ID: 586
Uniprot ID: BCAT1_HUMAN
Immunogen Region: 20-190
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 20-190 of human BCAT1 (NP_005495.2).
Immunogen Sequence: EVVGTFKAKDLIVTPATILK EKPDPNNLVFGTVFTDHMLT VEWSSEFGWEKPHIKPLQNL SLHPGSSALHYAVELFEGLK AFRGVDNKIRLFQPNLNMDR MYRSAVRATLPVFDKEELLE CIQQLVKLDQEWVPYSTSAS LYIRPTFIGTEPSLGVKKPT KALLFVLLSPV
Tissue Specificity During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.
Function Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.
Protein Name Branched-Chain-Amino-Acid Aminotransferase - Cytosolic
Bcat(C
Database Links Reactome: R-HSA-70895
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Branched-Chain-Amino-Acid Aminotransferase - Cytosolic antibody
Anti-Bcat(C antibody
Anti-BCAT1 antibody
Anti-BCT1 antibody
Anti-ECA39 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance