| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-BCAR3 (554-825) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BCAR3 |
| Gene ID: |
8412 |
| Uniprot ID: |
BCAR3_HUMAN |
| Immunogen Region: |
554-825 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 554-825 of human BCAR3 (NP_003558.1). |
| Immunogen Sequence: |
QHVLSMDCRVARILGVSEEM RRNMGVSSGLELITLPHGHQ LRLDIIERHNTMAIGIAVDI LGCTGTLEDRAATLSKIIQV AVELKDSMGDLYSFSALMKA LEMPQITRLEKTWTALRHQY TQTAILYEKQLKPFSKLLHE GRESTCVPPNNVSVPLLMPL VTLMERQAVTFEGTDMWEKN DQSCEIMLNHLATARFMAEA ADSYRMNAERILAGFQPDEE MNEICKTEFQMRLLWGSKG |
| Tissue Specificity | Ubiquitously expressed. Found in several cancer cell lines, but not in nonmalignant breast tissue. |
| Post Translational Modifications | Phosphorylated on tyrosine residues. |
| Function | Acts as an adapter protein downstream of several growth factor receptors to promote cell proliferation, migration, and redistribution of actin fibers. Specifically involved in INS/insulin signaling pathway by mediating MAPK1/ERK2-MAPK3/ERK1 activation and DNA synthesis. Promotes insulin-mediated membrane ruffling. In response to vasoconstrictor peptide EDN1, involved in the activation of RAP1 downstream of PTK2B via interaction with phosphorylated BCAR1. Inhibits cell migration and invasion via regulation of TGFB-mediated matrix digestion, actin filament rearrangement, and inhibition of invadopodia activity. May inhibit TGFB-SMAD signaling, via facilitating BCAR1 and SMAD2 and/or SMAD3 interaction. Regulates EGF-induced DNA synthesis. Required for the maintenance of ocular lens morphology and structural integrity, potentially via regulation of focal adhesion complex signaling. Acts upstream of PTPRA to regulate the localization of BCAR1 and PTPRA to focal adhesions, via regulation of SRC-mediated phosphorylation of PTPRA. Positively regulates integrin-induced tyrosine phosphorylation of BCAR1. Acts as a guanine nucleotide exchange factor (GEF) for small GTPases RALA, RAP1A and RRAS. However, in a contrasting study, lacks GEF activity towards RAP1. |
| Protein Name | Breast Cancer Anti-Estrogen Resistance Protein 3Novel Sh2-Containing Protein 2Sh2 Domain-Containing Protein 3b |
| Cellular Localisation | CytoplasmCell JunctionFocal AdhesionLocalization To Focal Adhesions Depends On Interaction With Ptpra |
| Alternative Antibody Names | Anti-Breast Cancer Anti-Estrogen Resistance Protein 3 antibodyAnti-Novel Sh2-Containing Protein 2 antibodyAnti-Sh2 Domain-Containing Protein 3b antibodyAnti-BCAR3 antibodyAnti-NSP2 antibodyAnti-SH2D3B antibodyAnti-UNQ271 antibodyAnti-PRO308 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Rig-I/DDX58 knockout (KO) A-549 cells, using [KO Validated] Rig-I/DDX58 Rabbit polyclonal antibody (STJ119976) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98761/382814/STJ119976_1__55820.1713151308.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Rig-I/DDX58 knockout (KO) A-549 cells, using [KO Validated] Rig-I/DDX58 Rabbit polyclonal antibody (STJ119976) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
