Anti-BACE1 antibody (452-501 aa) (STJ91795)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human BACE at the amino acid range 452-501
General Information
| Short Description | Rabbit polyclonal anti-Beta-secretase 1 (452-501 aa) for use in IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BACE1 |
| Gene ID | 23621 |
| Uniprot ID | BACE1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human BACE at the amino acid range 452-501 |
| Immunogen Region | 452-501 aa |
| Specificity | BACE Polyclonal Antibody detects endogenous levels of BACE protein. |
Additional Info
| Post Translational Modifications | N-Glycosylated. Addition of a bisecting N-acetylglucosamine by MGAT3 blocks lysosomal targeting, further degradation and is required for maintaining stability under stress conditions. Acetylated in the endoplasmic reticulum at Lys-126, Lys-275, Lys-279, Lys-285, Lys-299, Lys-300 and Lys-307. Acetylation by NAT8 and NAT8B is transient and deacetylation probably occurs in the Golgi. Acetylation regulates the maturation, the transport to the plasma membrane, the stability and the expression of the protein. Palmitoylation mediates lipid raft localization. Ubiquitinated at Lys-501, ubiquitination leads to lysosomal degradation. Monoubiquitinated and 'Lys-63'-linked polyubitinated. Deubiquitnated by USP8.inhibits lysosomal degradation. Phosphorylation at Ser-498 is required for interaction with GGA1 and retrograded transport from endosomal compartments to the trans-Golgi network. Non-phosphorylated BACE1 enters a direct recycling route to the cell surface. |
| Function | Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. Cleaves CHL1. |
| Protein Name | Beta-Secretase 1Aspartyl Protease 2Asp2Asp 2Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1Beta-Site App Cleaving Enzyme 1Memapsin-2Membrane-Associated Aspartic Protease 2 |
| Database Links | Reactome: R-HSA-977225 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinGolgi ApparatusTrans-Golgi NetworkEndoplasmic ReticulumEndosomeCell SurfaceCytoplasmic Vesicle MembraneMembrane RaftLysosomeLate EndosomeEarly EndosomeRecycling EndosomeCell ProjectionAxonDendritePredominantly Localized To The Later Golgi/Trans-Golgi Network (Tgn) And Minimally Detectable In The Early Golgi CompartmentsA Small Portion Is Also Found In The Endoplasmic ReticulumEndosomes And On The Cell SurfaceColocalization With App In Early Endosomes Is Due To Addition Of Bisecting N-Acetylglucosamine Which Blocks Targeting To Late Endosomes And LysosomesRetrogradly Transported From Endosomal Compartments To The Trans-Golgi Network In A Phosphorylation- And Gga1- Dependent Manner |
| Alternative Antibody Names | Anti-Beta-Secretase 1 antibodyAnti-Aspartyl Protease 2 antibodyAnti-Asp2 antibodyAnti-Asp 2 antibodyAnti-Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1 antibodyAnti-Beta-Site App Cleaving Enzyme 1 antibodyAnti-Memapsin-2 antibodyAnti-Membrane-Associated Aspartic Protease 2 antibodyAnti-BACE1 antibodyAnti-BACE antibodyAnti-KIAA1149 antibody |
Information sourced from Uniprot.org