Anti-Autophagy-related protein 12 antibody (N-Term) (STJA0040867)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeMixed
ImmunogenA synthetic peptide corresponding to the N-terminal of human ATG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis product list) has been used
General Information
| Short Description | Rabbit polyclonal anti-Autophagy-related protein 12 (N-Term) for use in IHC-F and WB in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-F/WB |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | Mixed |
| Conjugation | Unconjugated |
| Purification | Whole serum |
| Dilution Range | WB 1:200-1:1000IHC 1:200-1:1000ICC 1:200-1:1000 |
| Storage Instruction | Spin vial briefly before opening. Reconstitute in 100 ยตL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at-20ยฐC for a higher stability, and at 2-8ยฐC with an appropriate antibacterial |
Target Information
| Gene Symbol | ATG12 |
| Gene ID | 9140 |
| Uniprot ID | ATG12_HUMAN |
| Immunogen | A synthetic peptide corresponding to the N-terminal of human ATG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis product list) has been used |
| Immunogen Region | N-Term |
| Immunogen Sequence | Human |
| Specificity | IHC and WB confirmed the specificity for ATG12. Human, rat. |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Acetylated by EP300. |
| Function | Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. As part of the ATG8 conjugation system with ATG5 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress. (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established. |
| Protein Name | Ubiquitin-Like Protein Atg12Autophagy-Related Protein 12Apg12-Like |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-5205685Reactome: R-HSA-8934903Reactome: R-HSA-936440 |
| Cellular Localisation | CytoplasmPreautophagosomal Structure MembranePeripheral Membrane ProteinTecpr1 Recruits The Atg12-Atg5 Conjugate To The Autolysosomal Membrane |
| Alternative Antibody Names | Anti-Ubiquitin-Like Protein Atg12 antibodyAnti-Autophagy-Related Protein 12 antibodyAnti-Apg12-Like antibodyAnti-ATG12 antibodyAnti-APG12 antibodyAnti-APG12L antibody |
Information sourced from Uniprot.org