• Immunofluorescence analysis of U2OS cells using ATPIF1 antibody (STJ22734) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human trachea using ATPIF1 antibody (STJ22734) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human stomach using ATPIF1 antibody (STJ22734) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human liver cancer using ATPIF1 antibody (STJ22734) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using ATPIF1 antibody (STJ22734) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Anti-ATPIF1 antibody (STJ22734)

SKU:
STJ22734

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal anti-ATPIF1 antibody is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:100
IF: 1:50-1:100
Uniprot ID: ATIF1_HUMAN
Gene ID: 93974
Gene Symbol: ATP5IF1
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-106 of human ATPIF1 (NP_057395.1).
Post Translational Modifications Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid.
Function Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.
Protein Name Atpase Inhibitor - Mitochondrial
Atp Synthase F1 Subunit Epsilon
Inhibitor Of F(1f(O-Atpase
If(1
If1
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Atpase Inhibitor - Mitochondrial antibody
Anti-Atp Synthase F1 Subunit Epsilon antibody
Anti-Inhibitor Of F(1f(O-Atpase antibody
Anti-If(1 antibody
Anti-If1 antibody
Anti-ATP5IF1 antibody
Anti-ATPI antibody
Anti-ATPIF1 antibody

Information sourced from Uniprot.org

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