![Immunoprecipitation analysis of 300 Mu g extracts of K-562 cells using 3 Mu g [KO Validated] ATPIF1 rabbit polyclonal antibody (STJ22734). Western blot was performed from the immunoprecipitate using [KO Validated] ATPIF1 rabbit polyclonal antibody (STJ22734) at a dilition of 1:3000.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/99195/383900/STJ22734_IP_3__62700.1713152507.jpg?c=1 "Immunoprecipitation analysis of 300 Mu g extracts of K-562 cells using 3 Mu g [KO Validated] ATPIF1 rabbit polyclonal antibody (STJ22734). Western blot was performed from the immunoprecipitate using [KO Validated] ATPIF1 rabbit polyclonal antibody (STJ22734) at a dilition of 1:3000.")
Anti-ATPIF1 antibody (26-106) (STJ22734)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ATPIF1 (26-106) antibody for use in WB, IF, ICC, IP and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:100IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP5IF1 |
| Gene ID | 93974 |
| Uniprot ID | ATIF1_HUMAN |
| Immunogen Region | 26-106 |
| Immunogen Sequence | GSDQSENVDRGAGSIREAGG AFGKREQAEEERYFRAQSRE QLAALKKHHEEEIVHHKKEI ERLQKEIERHKQKIKMLKHD D |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 26-106 of human ATPIF1 (NP_057395.1). |
Additional Info
| Post Translational Modifications | Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid. |
| Function | Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme. |
| Protein Name | Atpase Inhibitor - MitochondrialAtp Synthase F1 Subunit EpsilonInhibitor Of F(1f(O-AtpaseIf(1If1 |
| Database Links | |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Atpase Inhibitor - Mitochondrial antibodyAnti-Atp Synthase F1 Subunit Epsilon antibodyAnti-Inhibitor Of F(1f(O-Atpase antibodyAnti-If(1 antibodyAnti-If1 antibodyAnti-ATP5IF1 antibodyAnti-ATPI antibodyAnti-ATPIF1 antibody |
Information sourced from Uniprot.org