• Immunohistochemistry analysis of ATP7B in paraffin-embedded human colon carcinoma tissue using ATP7B Rabbit polyclonal antibody (STJ27643) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ATP7B in paraffin-embedded rat liver tissue using ATP7B Rabbit polyclonal antibody (STJ27643) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ATP7B in paraffin-embedded human liver tissue using ATP7B Rabbit polyclonal antibody (STJ27643) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ATP7B in paraffin-embedded mouse kidney tissue using ATP7B Rabbit polyclonal antibody (STJ27643) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from Rat liver using ATP7B Rabbit polyclonal antibody (STJ27643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ATP7B antibody (1251-1354) (STJ27643)

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STJ27643

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP7B (1251-1354) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP7B
Gene ID: 540
Uniprot ID: ATP7B_HUMAN
Immunogen Region: 1251-1354
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1251-1354 of human ATP7B (NP_001230111.1).
Immunogen Sequence: SSVSVVLSSLQLKCYKKPDL ERYEAQAHGHMKPLTASQVS VHIGMDDRWRDSPRATPWDQ VSYVSQVSLSSLTSDKPSRH SAAADDDGDKWSLLLNGRDE E
Tissue Specificity Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues.
Post Translational Modifications Isoform 1 may be proteolytically cleaved at the N-terminus to produce the WND/140 kDa form.
Function Copper ion transmembrane transporter involved in the export of copper out of the cells. It is involved in copper homeostasis in the liver, where it ensures the efflux of copper from hepatocytes into the bile in response to copper overload.
Protein Name Copper-Transporting Atpase 2
Copper Pump 2
Wilson Disease-Associated Protein Cleaved Into - Wnd/140 Kda
Database Links Reactome: R-HSA-936837
Cellular Localisation Golgi Apparatus
Trans-Golgi Network Membrane
Multi-Pass Membrane Protein
Late Endosome
Predominantly Found In The Trans-Golgi Network (Tgn)
Localized In The Trans-Golgi Network Under Low Copper Conditions
Redistributes To Cytoplasmic Vesicles When Cells Are Exposed To Elevated Copper Levels
And Then Recycles Back To The Trans-Golgi Network When Copper Is Removed
Isoform 1: Golgi Apparatus Membrane
Isoform 2: Cytoplasm
Wnd/140 Kda: Mitochondrion
Alternative Antibody Names Anti-Copper-Transporting Atpase 2 antibody
Anti-Copper Pump 2 antibody
Anti-Wilson Disease-Associated Protein Cleaved Into - Wnd/140 Kda antibody
Anti-ATP7B antibody
Anti-PWD antibody
Anti-WC1 antibody
Anti-WND antibody

Information sourced from Uniprot.org

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