| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ATP6V1B1 |
| Gene ID: | 525 |
| Uniprot ID: | VATB1_HUMAN |
| Immunogen Region: | 1-280 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP6V1B1 (NP_001683.2). |
| Immunogen Sequence: | MAMEIDSRPGGLPGSSCNLG AAREHMQAVTRNYITHPRVT YRTVCSVNGPLVVLDRVKFA QYAEIVHFTLPDGTQRSGQV LEVAGTKAIVQVFEGTSGID ARKTTCEFTGDILRTPVSED MLGRVFNGSGKPIDKGPVVM AEDFLDINGQPINPHSRIYP EEMIQTGISPIDVMNSIARG QKIPIFSAAGLPHNEIAAQI CRQAGLVKKSKAVLDYHDDN FAIVFAAMGVNMETARFFK |
| Tissue Specificity | Kidney.localizes to early distal nephron, encompassing thick ascending limbs and distal convoluted tubules (at protein level). Expressed in the cochlea and endolymphatic sac. |
| Function | Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. Required for optimal olfactory function by mediating the acidification of the nasal olfactory epithelium. |
| Protein Name | V-Type Proton Atpase Subunit B - Kidney Isoform V-Atpase Subunit B 1 Endomembrane Proton Pump 58 Kda Subunit Vacuolar Proton Pump Subunit B 1 |
| Database Links | Reactome: R-HSA-1222556 Reactome: R-HSA-77387 Reactome: R-HSA-917977 Reactome: R-HSA-9639288 Reactome: R-HSA-983712 |
| Cellular Localisation | Apical Cell Membrane Basolateral Cell Membrane |
| Alternative Antibody Names | Anti-V-Type Proton Atpase Subunit B - Kidney Isoform antibody Anti-V-Atpase Subunit B 1 antibody Anti-Endomembrane Proton Pump 58 Kda Subunit antibody Anti-Vacuolar Proton Pump Subunit B 1 antibody Anti-ATP6V1B1 antibody Anti-ATP6B1 antibody Anti-VATB antibody Anti-VPP3 antibody |
Information sourced from Uniprot.org