• Western blot analysis of various lysates using ATP5J2 Rabbit polyclonal antibody (STJ119286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ATP5J2 antibody (1-94) (STJ119286)

SKU:
STJ119286

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP5J2 (1-94) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP5MF
Gene ID: 9551
Uniprot ID: ATPK_HUMAN
Immunogen Region: 1-94
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-94 of human ATP5J2 (NP_004880.1).
Immunogen Sequence: MASVGECPAPVPVKDKKLLE VKLGELPSWILMRDFSPSGI FGAFQRGYYRYYNKYINVKK GSISGITMVLACYVLFSYSF SYKHLKHERLRKYH
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
Protein Name Atp Synthase Subunit F - Mitochondrial
Atp Synthase Membrane Subunit F
Database Links Reactome: R-HSA-163210
Reactome: R-HSA-8949613
Cellular Localisation Mitochondrion
Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Atp Synthase Subunit F - Mitochondrial antibody
Anti-Atp Synthase Membrane Subunit F antibody
Anti-ATP5MF antibody
Anti-ATP5J2 antibody
Anti-ATP5JL antibody

Information sourced from Uniprot.org

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