• Western blot analysis of extracts of various cell lines, using ATP1B2 antibody (STJ111969) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.
  • Immunofluorescence analysis of C6 cells using ATP1B2 Rabbit polyclonal antibody (STJ111969) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ATP1B2 rabbit polyclonal antibody (STJ111969) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ATP1B2 antibody (STJ111969) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-ATP1B2 antibody (68-290) (STJ111969)

SKU:
STJ111969

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP1B2 (68-290) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP1B2
Gene ID: 482
Uniprot ID: AT1B2_HUMAN
Immunogen Region: 68-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 68-290 of human ATP1B2 (NP_001669.3).
Immunogen Sequence: DHTPKYQDRLATPGLMIRPK TENLDVIVNVSDTESWDQHV QKLNKFLEPYNDSIQAQKND VCRPGRYYEQPDNGVLNYPK RACQFNRTQLGNCSGIGDST HYGYSTGQPCVFIKMNRVIN FYAGANQSMNVTCAGKRDED AENLGNFVMFPANGNIDLMY FPYYGKKFHVNYTQPLVAVK FLNVTPNVEVNVECRINAAN IATDDERDKFAGRVAFKLRI NKT
Function This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known. Mediates cell adhesion of neurons and astrocytes, and promotes neurite outgrowth.
Protein Name Sodium/Potassium-Transporting Atpase Subunit Beta-2
Adhesion Molecule In Glia
Amog
Sodium/Potassium-Dependent Atpase Subunit Beta-2
Database Links Reactome: R-HSA-210991
Reactome: R-HSA-5578775
Reactome: R-HSA-936837
Reactome: R-HSA-9679191
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Sodium/Potassium-Transporting Atpase Subunit Beta-2 antibody
Anti-Adhesion Molecule In Glia antibody
Anti-Amog antibody
Anti-Sodium/Potassium-Dependent Atpase Subunit Beta-2 antibody
Anti-ATP1B2 antibody

Information sourced from Uniprot.org

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