| Host: |
Goat |
| Applications: |
WB/IHC-F/IHC-P |
| Reactivity: |
Human/Rat/Mouse/Monkey/Canine |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-ATPase Na+/K+ transporting subunit alpha 1 (100aa to N-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: |
This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: |
3 mg/ml |
| Dilution Range: |
WB 1:500-1:2000IHC-F 1:250-1:1000IHC-P 1:250-1:1000Ferreira JV Rosa Soares A Ramalho JS et al. PLoS One 2019 Oct. PMID: 31613922 |
| Storage Instruction: |
For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Gene Symbol: |
ATP1A1 |
| Gene ID: |
476 |
| Uniprot ID: |
AT1A1_HUMAN |
| Immunogen Region: |
100aa to N-Term |
| Accession Number: |
ENSG00000163399 |
| Specificity: |
Detects endogenous levels of ATP1a1 by Western blot in the whole cell lysates (HeLa, LS174T, SKOV3, etc.). |
| Immunogen: |
Purified recombinant peptide derived from within residues 100 aa to N-terminus of human ATP1a1 produced in E. coli. |
| Immunogen Sequence: |
MGKGVGRDKYEPAAVSEQGD KKGKKGKKDRDMDELKKEVS MDDHKLSLDELHRKYGTDLS RGLTSARAAEILARDGPNAL TPPPTTPEWIKFCRQLFGG |
| Post Translational Modifications | Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity. |
| Function | This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. |
| Protein Name | Sodium/Potassium-Transporting Atpase Subunit Alpha-1Na(+/K(+ Atpase Alpha-1 SubunitSodium Pump Subunit Alpha-1 |
| Database Links | Reactome: R-HSA-5578775Reactome: R-HSA-936837Reactome: R-HSA-9679191 |
| Cellular Localisation | Basolateral Cell MembraneMulti-Pass Membrane ProteinCell MembraneSarcolemmaCell ProjectionAxonMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Sodium/Potassium-Transporting Atpase Subunit Alpha-1 antibodyAnti-Na(+/K(+ Atpase Alpha-1 Subunit antibodyAnti-Sodium Pump Subunit Alpha-1 antibodyAnti-ATP1A1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
