Anti-ATOH8 antibody (150-250) (STJ119356)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ATOH8 (150-250) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATOH8 |
| Gene ID | 84913 |
| Uniprot ID | ATOH8_HUMAN |
| Immunogen Region | 150-250 |
| Immunogen Sequence | LRPRILLCAPPARPAPSAPP APPAPPESTVRPAPPTRPGE SSYSSISHVIYNNHQDSSAS PRKRPGEATAASSEIKALQQ TRRLLANARERTRVHTISAA F |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ATOH8 (NP_116216.2). |
Additional Info
| Tissue Specificity | Expressed in lung, liver, kidney, heart and pancreas. Expressed in endothel of umbilical vessels. |
| Function | Transcription factor that binds a palindromic (canonical) core consensus DNA sequence 5'-CANNTG- 3' known as an E-box element, possibly as a heterodimer with other bHLH proteins. Regulates endothelial cell proliferation, migration and tube-like structures formation. Modulates endothelial cell differentiation through NOS3. May be implicated in specification and differentiation of neuronal cell lineages in the brain. May participate in kidney development and may be involved in podocyte differentiation. During early embryonic development is involved in tissue-specific differentiation processes that are dependent on class II bHLH factors and namely modulates the differentiation program initiated by the pro-endocrine factor NEUROG3. During myogenesis, may play a role during the transition of myoblasts from the proliferative phase to the differentiation phase. Positively regulates HAMP transcription in two ways, firstly by acting directly on the HAMP promoter via E-boxes binding and indirectly through increased phosphorylation of SMAD protein complex. Repress NEUROG3-dependent gene activation in a gene-specific manner through at least two mechanisms.requires only either the sequestering of a general partner such as TCF3 through heterodimerization, either also requires binding of the bHLH domain to DNA via a basic motif. |
| Protein Name | Transcription Factor Atoh8Class A Basic Helix-Loop-Helix Protein 21Bhlha21Helix-Loop-Helix Protein Hath-6Hath6Protein Atonal Homolog 8 |
| Database Links | |
| Cellular Localisation | NucleusNucleus SpeckleCytoplasm |
| Alternative Antibody Names | Anti-Transcription Factor Atoh8 antibodyAnti-Class A Basic Helix-Loop-Helix Protein 21 antibodyAnti-Bhlha21 antibodyAnti-Helix-Loop-Helix Protein Hath-6 antibodyAnti-Hath6 antibodyAnti-Protein Atonal Homolog 8 antibodyAnti-ATOH8 antibodyAnti-ATH6 antibodyAnti-BHLHA21 antibody |
Information sourced from Uniprot.org