Anti-ATG9B antibody (715-924) (STJ29543)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ATG9B (715-924) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATG9B |
| Gene ID | 285973 |
| Uniprot ID | ATG9B_HUMAN |
| Immunogen Region | 715-924 |
| Immunogen Sequence | HPLWRPPGHSSKFLGHLWGR VQQDAAAWGATSARGPSTPG VLSNCTSPLPEAFLANLFVH PLLPPRDLSPTAPCPAAATA SLLASISRIAQDPSSVSPGG TGGQKLAQLPELASAEMSLH VIYLHQLHQQQQQQEPWGEA AASILSRPCSSPSQPPSPDE EKPSWSSDGSSPASSPRQQW GTQKARNLFPGGFQVTTDTQ KEPDRASCTD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 715-924 of human ATG9B (NP_001303985.1). |
Additional Info
| Tissue Specificity | Highly expressed in placenta (trophoblast cells) and pituitary gland. Not expressed in vascular endothelial. |
| Function | Phospholipid scramblase involved in autophagy by mediating autophagosomal membrane expansion. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 (ATG2A or ATG2B) from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion. In addition to autophagy, also plays a role in necrotic cell death. |
| Protein Name | Autophagy-Related Protein 9bApg9-Like 2Nitric Oxide Synthase 3-Overlapping Antisense Gene ProteinProtein Sone |
| Database Links | Reactome: R-HSA-1632852 |
| Cellular Localisation | Preautophagosomal Structure MembraneMulti-Pass Membrane ProteinUnder Amino Acid Starvation Or Rapamycin TreatmentRedistributes From A Juxtanuclear Clustered Pool To A Dispersed Peripheral Cytosolic PoolThe Starvation-Induced Redistribution Depends On Ulk1 And Atg13 |
| Alternative Antibody Names | Anti-Autophagy-Related Protein 9b antibodyAnti-Apg9-Like 2 antibodyAnti-Nitric Oxide Synthase 3-Overlapping Antisense Gene Protein antibodyAnti-Protein Sone antibodyAnti-ATG9B antibodyAnti-APG9L2 antibodyAnti-NOS3AS antibody |
Information sourced from Uniprot.org