• Western blot analysis of various lysates, using ATG13 antibody (STJ22706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 25s.
  • Immunofluorescence analysis of HeLa cells using ATG13 rabbit polyclonal antibody (STJ22706) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ATG13 rabbit polyclonal antibody (STJ22706) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG13 antibody (281-517) (STJ22706)

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STJ22706

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATG13 (281-517) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATG13
Gene ID: 9776
Uniprot ID: ATG13_HUMAN
Immunogen Region: 281-517
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 281-517 of human ATG13 (NP_001136145.1).
Immunogen Sequence: ADLAYPVVFAAGLNATHPHQ LMVPGKEGGVPLAPNQPVHG TQADQERLATCTPSDRTHCA ATPSSSEDTETVSNSSEGRA SPHDVLETIFVRKVGAFVNK PINQVTLTSLDIPFAMFAPK NLELEDTDPMVNPPDSPETE SPLQGSLHSDGSSGGSSGNT HDDFVMIDFKPAFSKDDILP MDLGTFYREFQNPPQLSSLS
Post Translational Modifications Phosphorylated by ULK1, ULK2 and mTOR. Phosphorylation status depends on nutrient-rich conditions.dephosphorylated during starvation or following treatment with rapamycin. ULK1-mediated phosphorylation of ATG13 at Ser-355 is required for efficient clearance of depolarized mitochondria.
Function Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.
Protein Name Autophagy-Related Protein 13
Database Links Reactome: R-HSA-1632852
Cellular Localisation Cytoplasm
Cytosol
Preautophagosomal Structure
Under Starvation Conditions
Is Localized To Puncate Structures Primarily Representing The Isolation Membrane
The Isolation Membrane Sequesters A Portion Of The Cytoplasm Resulting In Autophagosome Formation
Alternative Antibody Names Anti-Autophagy-Related Protein 13 antibody
Anti-ATG13 antibody
Anti-KIAA0652 antibody

Information sourced from Uniprot.org

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