• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g ATF1 antibody (STJ28355). Western blot was performed from the immunoprecipitate using ATF1 antibody (STJ28355) at a dilution of 1:1000.
  • Western blot analysis of lysates from HeLa cells, using ATF1 Rabbit polyclonal antibody (STJ28355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ATF1 antibody (1-210) (STJ28355)

SKU:
STJ28355

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATF1 (1-210) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATF1
Gene ID: 466
Uniprot ID: ATF1_HUMAN
Immunogen Region: 1-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human ATF1 (NP_005162.1).
Immunogen Sequence: MEDSHKSTTSETAPQPGSAV QGAHISHIAQQVSSLSESEE SQDSSDSIGSSQKAHGILAR RPSYRKILKDLSSEDTRGRK GDGENSGVSAAVTSMSVPTP IYQTSSGQYIAIAPNGALQL ASPGTDGVQGLQTLTMTNSG STQQGTTILQYAQTSDGQQI LVPSNQVVVQTASGDMQTYQ IRTTPSATSLPQTVVMTSPV TLTSQTTKTD
Post Translational Modifications Phosphorylated at Ser-198 by HIPK2 in response to genotoxic stress. This phosphorylation promotes transcription repression of FTH1 and other antioxidant detoxification genes. The CDK3-mediated phosphorylation at Ser-63 promotes its transactivation and transcriptional activities. Phosphorylated at Ser-63 by RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli.
Function This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGTACAG-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.
Protein Name Cyclic Amp-Dependent Transcription Factor Atf-1
Camp-Dependent Transcription Factor Atf-1
Activating Transcription Factor 1
Protein Treb36
Database Links Reactome: R-HSA-199920
Reactome: R-HSA-9031628
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Cyclic Amp-Dependent Transcription Factor Atf-1 antibody
Anti-Camp-Dependent Transcription Factor Atf-1 antibody
Anti-Activating Transcription Factor 1 antibody
Anti-Protein Treb36 antibody
Anti-ATF1 antibody

Information sourced from Uniprot.org

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