| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse/Rat/Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ASIC1 (460-505 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1:1000 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
ASIC1 |
| Gene ID: |
41 |
| Uniprot ID: |
ASIC1_HUMAN |
| Immunogen Region: |
460-505 aa |
| Specificity: |
Specific for AISC1. |
| Immunogen: |
A synthetic peptide from aa region 460-505 of human ASIC1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse. |
| Post Translational Modifications | Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel. |
| Function | Isoform 2 and isoform 3 function as proton-gated sodium channels.they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear. Isoform 1 does not display proton-gated cation channel activity. |
| Protein Name | Acid-Sensing Ion Channel 1Asic1Amiloride-Sensitive Cation Channel 2 - NeuronalBrain Sodium Channel 2Bnac2 |
| Database Links | Reactome: R-HSA-2672351 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinLocalizes In Synaptosomes At Dendritic Synapses Of NeuronsColocalizes With Dlg4 |
| Alternative Antibody Names | Anti-Acid-Sensing Ion Channel 1 antibodyAnti-Asic1 antibodyAnti-Amiloride-Sensitive Cation Channel 2 - Neuronal antibodyAnti-Brain Sodium Channel 2 antibodyAnti-Bnac2 antibodyAnti-ASIC1 antibodyAnti-ACCN2 antibodyAnti-BNAC2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
