• Immunofluorescence analysis of C6 cells using ARX Rabbit polyclonal antibody (STJ119416) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ARX in paraffin-embedded Rat testis using ARX Rabbit polyclonal antibody (STJ119416) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ARX in paraffin-embedded Rat testis using ARX Rabbit polyclonal antibody (STJ119416) at dilution of 1:50 (20x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ARX Rabbit polyclonal antibody (STJ119416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ARX antibody (C-Term) (STJ119416)

SKU:
STJ119416

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARX (C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARX
Gene ID: 170302
Uniprot ID: ARX_HUMAN
Immunogen Region: C-Term
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human ARX (NP_620689.1).
Immunogen Sequence: LLRQPTPAVEGAVASGALAD PATAAADRRASSIAALRLKA KEHAAQLTQLNILPGTSTGK EVC
Tissue Specificity Expressed predominantly in fetal and adult brain and skeletal muscle. Expression is specific to the telencephalon and ventral thalamus. There is an absence of expression in the cerebellum throughout development and also in adult.
Function Transcription factor. Binds to specific sequence motif 5'-TAATTA-3' in regulatory elements of target genes, such as histone demethylase KDM5C. Positively modulates transcription of KDM5C. Activates expression of KDM5C synergistically with histone lysine demethylase PHF8 and perhaps in competition with transcription regulator ZNF711.synergy may be related to enrichment of histone H3K4me3 in regulatory elements. Required for normal brain development. Plays a role in neuronal proliferation, interneuronal migration and differentiation in the embryonic forebrain. May also be involved in axonal guidance in the floor plate.
Protein Name Homeobox Protein Arx
Aristaless-Related Homeobox
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Arx antibody
Anti-Aristaless-Related Homeobox antibody
Anti-ARX antibody

Information sourced from Uniprot.org

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