• Western blot analysis of C2C12, using ARID1A Rabbit polyclonal antibody (STJ11100580) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-ARID1A antibody (2195-2285) (STJ11100580)

SKU:
STJ11100580

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARID1A (2195-2285) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARID1A
Gene ID: 8289
Uniprot ID: ARI1A_HUMAN
Immunogen Region: 2195-2285
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2195-2285 of human ARID1A (NP_006006.3).
Immunogen Sequence: LLGFLEDSLAATQFQQSQAS LLHMQNPPFEPTSVDMMRRA ARALLALAKVDENHSEFTLY ESRLLDISVSPLMNSLVSQV ICDVLFLIGQS
Tissue Specificity Highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and PBL, and at a much lower level in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
Function Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Protein Name At-Rich Interactive Domain-Containing Protein 1a
Arid Domain-Containing Protein 1a
B120
Brg1-Associated Factor 250
Baf250
Brg1-Associated Factor 250a
Baf250a
Osa Homolog 1
Hosa1
Swi-Like Protein
Swi/Snf Complex Protein P270
Swi/Snf-Related - Matrix-Associated - Actin-Dependent Regulator Of Chromatin Subfamily F Member 1
Held
Database Links Reactome: R-HSA-3214858
Reactome: R-HSA-8939243
Cellular Localisation Nucleus
Alternative Antibody Names Anti-At-Rich Interactive Domain-Containing Protein 1a antibody
Anti-Arid Domain-Containing Protein 1a antibody
Anti-B120 antibody
Anti-Brg1-Associated Factor 250 antibody
Anti-Baf250 antibody
Anti-Brg1-Associated Factor 250a antibody
Anti-Baf250a antibody
Anti-Osa Homolog 1 antibody
Anti-Hosa1 antibody
Anti-Swi-Like Protein antibody
Anti-Swi/Snf Complex Protein P270 antibody
Anti-Swi/Snf-Related - Matrix-Associated - Actin-Dependent Regulator Of Chromatin Subfamily F Member 1 antibody
Anti-Held antibody
Anti-ARID1A antibody
Anti-BAF250 antibody
Anti-BAF250A antibody
Anti-C1orf4 antibody
Anti-OSA1 antibody
Anti-SMARCF1 antibody

Information sourced from Uniprot.org

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