Anti-ARHGEF4 antibody (270-350 aa) (STJ191327)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 270-350
General Information
| Short Description | Rabbit polyclonal anti-Rho guanine nucleotide exchange factor 4 (270-350 aa) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ARHGEF4 |
| Gene ID | 50649 |
| Uniprot ID | ARHG4_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 270-350 |
| Immunogen Region | 270-350 aa |
| Specificity | ARHG4 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Function | Acts as a guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression. |
| Protein Name | Rho Guanine Nucleotide Exchange Factor 4Apc-Stimulated Guanine Nucleotide Exchange Factor 1AsefAsef1 |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013148Reactome: R-HSA-9013149 |
| Cellular Localisation | Isoform 3: CytoplasmCell ProjectionRuffle MembranePeripheral Membrane ProteinCytoplasmic SideAssociated With Membrane Ruffles |
| Alternative Antibody Names | Anti-Rho Guanine Nucleotide Exchange Factor 4 antibodyAnti-Apc-Stimulated Guanine Nucleotide Exchange Factor 1 antibodyAnti-Asef antibodyAnti-Asef1 antibodyAnti-ARHGEF4 antibodyAnti-KIAA1112 antibody |
Information sourced from Uniprot.org