| Tissue Specificity | Ubiquitously expressed. |
| Post Translational Modifications | Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2. |
| Function | Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. In lymphoid follicles, may trigger activation of GNA13 as part of S1PR2-dependent signaling pathway that leads to inhibition of germinal center (GC) B cell growth and migration outside the GC niche. |
| Protein Name | Rho Guanine Nucleotide Exchange Factor 1115 Kda Guanine Nucleotide Exchange FactorP115-RhogefP115rhogefSub1.5 |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106 |
| Cellular Localisation | CytoplasmMembraneTranslocated To The Membrane By Activated Gna13 Or Lpa Stimulation |
| Alternative Antibody Names | Anti-Rho Guanine Nucleotide Exchange Factor 1 antibodyAnti-115 Kda Guanine Nucleotide Exchange Factor antibodyAnti-P115-Rhogef antibodyAnti-P115rhogef antibodyAnti-Sub1.5 antibodyAnti-ARHGEF1 antibody |
Information sourced from Uniprot.org
![Immunohistochemistry analysis of ARHGEF1 in paraffin-embedded human tonsil using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95232/377118/STJ116239_1__44864.1713144353.jpg?c=1 "Immunohistochemistry analysis of ARHGEF1 in paraffin-embedded human tonsil using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and ARHGEF1 knockout (KO) 293T cells, using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95232/377119/STJ116239_2__74076.1713144354.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ARHGEF1 knockout (KO) 293T cells, using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of various lysates using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95232/377120/STJ116239_3__99060.1713144354.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry analysis of ARHGEF1 in paraffin-embedded human tonsil using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/95232/377118/STJ116239_1__44864.1713144353.jpg?c=1 "Immunohistochemistry analysis of ARHGEF1 in paraffin-embedded human tonsil using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
