![Immunohistochemistry analysis of ARHGEF1 in paraffin-embedded human tonsil using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95232/377118/STJ116239_1__44864.1713144353.jpg?c=1 "Immunohistochemistry analysis of ARHGEF1 in paraffin-embedded human tonsil using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and ARHGEF1 knockout (KO) 293T cells, using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95232/377119/STJ116239_2__74076.1713144354.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ARHGEF1 knockout (KO) 293T cells, using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of various lysates using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95232/377120/STJ116239_3__99060.1713144354.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry analysis of ARHGEF1 in paraffin-embedded human tonsil using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/95232/377118/STJ116239_1__44864.1713144353.jpg?c=1 "Immunohistochemistry analysis of ARHGEF1 in paraffin-embedded human tonsil using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:100 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ARHGEF1 |
| Gene ID: | 9138 |
| Uniprot ID: | ARHG1_HUMAN |
| Immunogen Region: | 783-912 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 783-912 of human ARHGEF1 (NP_004697.2). |
| Immunogen Sequence: | PLLSSSENGNGGRETSPADA RTERILSDLLPFCRPGPEGQ LAATALRKVLSLKQLLFPAE EDNGAGPPRDGDGVPGGGPL SPARTQEIQENLLSLEETMK QLEELEEEFCRLRPLLSQLG GNSVPQPGCT |
| Tissue Specificity | Ubiquitously expressed. |
| Post Translational Modifications | Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2. |
| Function | Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. |
| Protein Name | Rho Guanine Nucleotide Exchange Factor 1 115 Kda Guanine Nucleotide Exchange Factor P115-Rhogef P115rhogef Sub1.5 |
| Database Links | Reactome: R-HSA-193648 Reactome: R-HSA-416482 Reactome: R-HSA-8980692 Reactome: R-HSA-9013026 Reactome: R-HSA-9013106 |
| Cellular Localisation | Cytoplasm Membrane Translocated To The Membrane By Activated Gna13 Or Lpa Stimulation |
| Alternative Antibody Names | Anti-Rho Guanine Nucleotide Exchange Factor 1 antibody Anti-115 Kda Guanine Nucleotide Exchange Factor antibody Anti-P115-Rhogef antibody Anti-P115rhogef antibody Anti-Sub1.5 antibody Anti-ARHGEF1 antibody |
Information sourced from Uniprot.org