| Host: | Rabbit |
| Applications: | WB/ELISA/IHC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Rho guanine nucleotide exchange factor 1 (162-211 aa) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ARHGEF1 |
| Gene ID: | 9138 |
| Uniprot ID: | ARHG1_HUMAN |
| Immunogen Region: | 162-211 aa |
| Specificity: | RhoGEF p115 Polyclonal Antibody detects endogenous levels of RhoGEF p115 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human ARHGEF1 at the amino acid range 162-211 |
| Post Translational Modifications | Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2. |
| Function | Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. |
| Protein Name | Rho Guanine Nucleotide Exchange Factor 1115 Kda Guanine Nucleotide Exchange FactorP115-RhogefP115rhogefSub1.5 |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106 |
| Cellular Localisation | CytoplasmMembraneTranslocated To The Membrane By Activated Gna13 Or Lpa Stimulation |
| Alternative Antibody Names | Anti-Rho Guanine Nucleotide Exchange Factor 1 antibodyAnti-115 Kda Guanine Nucleotide Exchange Factor antibodyAnti-P115-Rhogef antibodyAnti-P115rhogef antibodyAnti-Sub1.5 antibodyAnti-ARHGEF1 antibody |
Information sourced from Uniprot.org
