• Western blot analysis of lysates from A549 cells, using ARFGEF2 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of A549 cells, using ARFGEF2 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of various cells using BIG2 Polyclonal Antibody

Anti-ARFGEF2 antibody (1491-1540 aa) (STJ91854)

SKU:
STJ91854

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Brefeldin A-inhibited guanine nucleotide-exchange protein 2 (1491-1540 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARFGEF2
Gene ID: 10564
Uniprot ID: BIG2_HUMAN
Immunogen Region: 1491-1540 aa
Specificity: BIG2 Polyclonal Antibody detects endogenous levels of BIG2 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ARFGEF2 at the amino acid range 1491-1540
Post Translational Modifications In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1.
Function Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles.the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways.
Protein Name Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein 2
Brefeldin A-Inhibited Gep 2
Adp-Ribosylation Factor Guanine Nucleotide-Exchange Factor 2
Database Links Reactome: R-HSA-390471
Cellular Localisation Cytoplasm
Membrane
Golgi Apparatus
Perinuclear Region
Trans-Golgi Network
Endosome
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cell Projection
Dendrite
Cytoplasmic Vesicle
Synapse
Translocates From Cytoplasm To Membranes Upon Camp Treatment
Localized In Recycling Endosomes
Alternative Antibody Names Anti-Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein 2 antibody
Anti-Brefeldin A-Inhibited Gep 2 antibody
Anti-Adp-Ribosylation Factor Guanine Nucleotide-Exchange Factor 2 antibody
Anti-ARFGEF2 antibody
Anti-ARFGEP2 antibody
Anti-BIG2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance