• Western blot analysis of lysates from Mouse brain, using Amphiregulin Rabbit polyclonal antibody (STJ22665) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 300s.
  • Western blot analysis of lysates from A-549 cells, using Amphiregulin Rabbit polyclonal antibody (STJ22665) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-AREG antibody (20-252) (STJ22665)

SKU:
STJ22665

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Amphiregulin (20-252) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AREG
Gene ID: 374
Uniprot ID: AREG_HUMAN
Immunogen Region: 20-252
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-252 of human Amphiregulin (NP_001648.1).
Immunogen Sequence: SGHYAAGLDLNDTYSGKREP FSGDHSADGFEVTSRSEMSS GSEISPVSEMPSSSEPSSGA DYDYSEEYDNEPQIPGYIVD DSVRVEQVVKPPQNKTESEN TSDKPKRKKKGGKNGKNRRN RKKKNPCNAEFQNFCIHGEC KYIEHLEAVTCKCQQEYFGE RCGEKSMKTHSMIDSSLSKI ALAAIAAFMSAVILTAVAVI TVQLRRQYVRKYEGEAEERK KLRQENGNVHAIA
Function Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
Protein Name Amphiregulin
Ar
Colorectum Cell-Derived Growth Factor
Crdgf
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-177929
Reactome: R-HSA-179812
Reactome: R-HSA-180292
Reactome: R-HSA-180336
Reactome: R-HSA-182971
Reactome: R-HSA-204005
Reactome: R-HSA-212718
Reactome: R-HSA-2219530
Reactome: R-HSA-5638303
Reactome: R-HSA-5673001
Reactome: R-HSA-5694530
Reactome: R-HSA-6811558
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-9009391
Reactome: R-HSA-9634638
Cellular Localisation Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Amphiregulin antibody
Anti-Ar antibody
Anti-Colorectum Cell-Derived Growth Factor antibody
Anti-Crdgf antibody
Anti-AREG antibody
Anti-AREGB antibody
Anti-SDGF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance