• Confocal imaging of paraffin-embedded Human liver cancer tissue using ApoM Rabbit monoclonal antibody (STJ11102273, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of Hep G2 cells using ApoM Rabbit monoclonal antibody (STJ11102273, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of various lysates using ApoM Rabbit monoclonal antibody (STJ11102273) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-APOM antibody (1-100) [S3MR] (STJ11102273)

SKU:
STJ11102273

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ApoM (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOM
Gene ID: 55937
Uniprot ID: APOM_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ApoM (O95445).
Immunogen Sequence: MFHQIWAALLYFYGIILNSI YQCPEHSQLTTLGVDGKEFP EVHLGQWYFIAGAAPTKEEL ATFDPVDNIVFNMAAGSAPM QLHLRATIRMKDGLCVPRKW
Tissue Specificity Plasma protein. Expressed in liver and kidney.
Function Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
Protein Name Apolipoprotein M
Apo-M
Apom
Protein G3a
Database Links Reactome: R-HSA-975634
Cellular Localisation Secreted
Present In High Density Lipoprotein (Hdl) And To A Lesser Extent In Triglyceride-Rich Lipoproteins (Tgrlp) And Low Density Lipoproteins (Ldl)
Alternative Antibody Names Anti-Apolipoprotein M antibody
Anti-Apo-M antibody
Anti-Apom antibody
Anti-Protein G3a antibody
Anti-APOM antibody
Anti-G3A antibody
Anti-NG20 antibody
Anti-HSPC336 antibody

Information sourced from Uniprot.org

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