• Western blot analysis of extracts of various cell lines, using APOL1 rabbit monoclonal antibody (STJ11102075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunofluorescence analysis of HepG2 cells using APOL1 rabbit monoclonal antibody (STJ11102075) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-APOL1 antibody [ARC1124] (STJ11102075)

SKU:
STJ11102075

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Host: Rabbit
Applications: WB, IF
Reactivity: Human, Mouse
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit monoclonal antibody anti-APOL1 is suitable for use in Western Blot and Immunofluorescence.
Clonality: Monoclonal
Clone ID: ARC1124
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: APOL1
Gene ID: 8542
Uniprot ID: APOL1_HUMAN
Immunogen: A synthesized peptide derived from human APOL1
Tissue Specificity Plasma. Found on APOA-I-containing high density lipoprotein (HDL3). Expressed in pancreas, lung, prostate, liver, placenta and spleen.
Post Translational Modifications Phosphorylated by FAM20C in the extracellular medium.
Function May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.
Protein Name Apolipoprotein L1
Apolipoprotein L
Apo-L
Apol
Apolipoprotein L-I
Apol-I
Database Links Reactome: R-HSA-2168880
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative Antibody Names Anti-Apolipoprotein L1 antibody
Anti-Apolipoprotein L antibody
Anti-Apo-L antibody
Anti-Apol antibody
Anti-Apolipoprotein L-I antibody
Anti-Apol-I antibody
Anti-APOL1 antibody
Anti-APOL antibody

Information sourced from Uniprot.org

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