• Confocal imaging of Hep G2 cells using APOL1 Rabbit monoclonal antibody (STJ11102075, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of various lysates using APOL1 Rabbit monoclonal antibody (STJ11102075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-APOL1 antibody (303-398) [S5MR] (STJ11102075)

SKU:
STJ11102075

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-APOL1 (303-398) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOL1
Gene ID: 8542
Uniprot ID: APOL1_HUMAN
Immunogen Region: 303-398
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 303-398 of human APOL1 (O14791).
Immunogen Sequence: RPRVTEPISAESGEQVERVN EPSILEMSRGVKLTDVAPVS FFLVLDVVYLVYESKHLHEG AKSETAEELKKVAQELEEKL NILNNNYKILQADQEL
Tissue Specificity Plasma. Found on APOA-I-containing high density lipoprotein (HDL3). Expressed in pancreas, lung, prostate, liver, placenta and spleen.
Post Translational Modifications Phosphorylated by FAM20C in the extracellular medium.
Function May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.
Protein Name Apolipoprotein L1
Apolipoprotein L
Apo-L
Apol
Apolipoprotein L-I
Apol-I
Database Links Reactome: R-HSA-2168880
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative Antibody Names Anti-Apolipoprotein L1 antibody
Anti-Apolipoprotein L antibody
Anti-Apo-L antibody
Anti-Apol antibody
Anti-Apolipoprotein L-I antibody
Anti-Apol-I antibody
Anti-APOL1 antibody
Anti-APOL antibody

Information sourced from Uniprot.org

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