Host: | Goat |
Applications: | Pep-ELISA/IHC |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Goat polyclonal antibody anti-APOBEC3G/ARP9 (Internal) is suitable for use in ELISA and Immunohistochemistry research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: | 0.5 mg/mL |
Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000. IHC: In paraffin embedded Human Kidney shows weak cytoplasm staining and stronger nuclear staining in a selection of glomerular cells. Also nuclear membrane staining elsewhere. Recommended |
Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: | APOBEC3G |
Gene ID: | 60489 |
Uniprot ID: | ABC3G_HUMAN |
Immunogen Region: | Internal |
Accession Number: | NP_068594.1 |
Immunogen Sequence: | SKWRKLHRDQE |
Post Translational Modifications | (Microbial infection) Following infection by HIV-1, ubiquitinated by a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) hijacked by the HIV-1 Vif protein, leading to its degradation. Deubiquitinated by USP49.leading to stabilization. Phosphorylation at Thr-32 reduces its binding to HIV-1 Vif and subsequent ubiquitination and degradation thus promoting its antiviral activity. |
Function | DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits potent antiviral activity against Vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity also against simian immunodeficiency viruses (SIVs), hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV). May inhibit the mobility of LTR and non-LTR retrotransposons. |
Protein Name | Dna Dc->Du-Editing Enzyme Apobec-3g Apobec-Related Cytidine Deaminase Apobec-Related Protein Arcd Apobec-Related Protein 9 Arp-9 Cem-15 Cem15 Deoxycytidine Deaminase A3g |
Database Links | Reactome: R-HSA-180585 Reactome: R-HSA-180689 |
Cellular Localisation | Cytoplasm Nucleus P-Body Mainly Cytoplasmic Small Amount Are Found In The Nucleus During Hiv-1 Infection Virion-Encapsidated In Absence Of Hiv-1 Vif |
Alternative Antibody Names | Anti-Dna Dc->Du-Editing Enzyme Apobec-3g antibody Anti-Apobec-Related Cytidine Deaminase antibody Anti-Apobec-Related Protein antibody Anti-Arcd antibody Anti-Apobec-Related Protein 9 antibody Anti-Arp-9 antibody Anti-Cem-15 antibody Anti-Cem15 antibody Anti-Deoxycytidine Deaminase antibody Anti-A3g antibody Anti-APOBEC3G antibody Anti-MDS019 antibody |
Information sourced from Uniprot.org