• Western blot analysis of extracts of various cell lines, using APLP1 rabbit polyclonal antibody (STJ28950) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using APLP1 rabbit polyclonal antibody (STJ28950) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using APLP1 rabbit polyclonal antibody (STJ28950) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of Neuro-2a cells using APLP1 rabbit polyclonal antibody (STJ28950) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of SH-SY5Y cells using APLP1 rabbit polyclonal antibody (STJ28950) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-APLP1 antibody (285-490) (STJ28950)

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STJ28950

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APLP1 (285-490) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APLP1
Gene ID: 333
Uniprot ID: APLP1_HUMAN
Immunogen Region: 285-490
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 285-490 of human APLP1 (NP_005157.1).
Immunogen Sequence: TPTPRPTDGVDIYFGMPGEI SEHEGFLRAKMDLEERRMRQ INEVMREWAMADNQSKNLPK ADRQALNEHFQSILQTLEEQ VSGERQRLVETHATRVIALI NDQRRAALEGFLAALQADPP QAERVLLALRRYLRAEQKEQ RHTLRHYQHVAAVDPEKAQQ MRFQVHTHLQVIEERVNQSL GLLDQNPHLAQELRPQIQEL LHSEHL
Tissue Specificity Expressed in the cerebral cortex where it is localized to the postsynaptic density (PSD).
Post Translational Modifications Proteolytically cleaved by caspases during neuronal apoptosis. Cleaved, in vitro, at Asp-620 by caspase-3. N- and O-glycosylated. O-glycosylation with core 1 or possibly core 8 glycans. Glycosylation on Ser-227 is the preferred site to Thr-228.
Function May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding. Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I. The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis.
Protein Name Amyloid Beta Precursor Like Protein 1
Amyloid Beta
A4 Precursor-Like Protein 1
Amyloid-Like Protein 1
Aplp
Aplp-1 Cleaved Into - C30
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
C30: Cytoplasm
C-Terminally Processed In The Golgi Complex
Alternative Antibody Names Anti-Amyloid Beta Precursor Like Protein 1 antibody
Anti-Amyloid Beta antibody
Anti-A4 Precursor-Like Protein 1 antibody
Anti-Amyloid-Like Protein 1 antibody
Anti-Aplp antibody
Anti-Aplp-1 Cleaved Into - C30 antibody
Anti-APLP1 antibody

Information sourced from Uniprot.org

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