| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-APLF (200-380) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
APLF |
| Gene ID: |
200558 |
| Uniprot ID: |
APLF_HUMAN |
| Immunogen Region: |
200-380 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 200-380 of human APLF (NP_775816.1). |
| Immunogen Sequence: |
LSVPAISGGNVIQGSGKEEI CKDKSQLNTTQQGRRQLISS GSSENTSAEQDTGEECKNTD QEESTISSKEMPQSFSAITL SNTEMNNIKTNAQRNKLPIE ELGKVSKHKIATKRTPHKED EAMSCSENCSSAQGDSLQDE SQGSHSESSSNPSNPETLHA KATDSVLQGSEGNKVKRTSC M |
| Post Translational Modifications | Poly-ADP-ribosylated. In addition to binding non covalently poly-ADP-ribose via its PBZ-type zinc fingers, the protein is also covalently poly-ADP-ribosylated by PARP1. Phosphorylated in an ATM-dependent manner upon double-strand DNA break. |
| Function | Histone chaperone involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with branched poly-ADP-ribose chains, a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Following recruitment to DNA damage sites, acts as a histone chaperone that mediates histone eviction during DNA repair and promotes recruitment of histone variant MACROH2A1. Also has a nuclease activity: displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ). Also acts as a negative regulator of cell pluripotency by promoting histone exchange. Required for the embryo implantation during the epithelial to mesenchymal transition in females. |
| Protein Name | Aprataxin And Pnk-Like FactorApurinic-Apyrimidinic Endonuclease AplfPnk And Aptx-Like Fha Domain-Containing ProteinXrcc1-Interacting Protein 1 |
| Cellular Localisation | NucleusChromosomeCytoplasmCytosolLocalizes To Dna Damage SitesAccumulates At Single-Strand Breaks And Double-Strand Breaks Via The Pbz-Type Zinc Fingers |
| Alternative Antibody Names | Anti-Aprataxin And Pnk-Like Factor antibodyAnti-Apurinic-Apyrimidinic Endonuclease Aplf antibodyAnti-Pnk And Aptx-Like Fha Domain-Containing Protein antibodyAnti-Xrcc1-Interacting Protein 1 antibodyAnti-APLF antibodyAnti-C2orf13 antibodyAnti-PALF antibodyAnti-XIP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and PRMT8 knockout (KO) 293T cells, using [KO Validated] PRMT8 Rabbit polyclonal antibody (STJ11100394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89456/357967/STJ11100394_1__99373.1713122215.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PRMT8 knockout (KO) 293T cells, using [KO Validated] PRMT8 Rabbit polyclonal antibody (STJ11100394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
