• Western blot analysis of extracts of mouse testis, using APAF1 antibody (STJ117996) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using APAF1 antibody (STJ117996) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Immunofluorescence analysis of A-549 cells using APAF1 rabbit polyclonal antibody (STJ117996) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using APAF1 rabbit polyclonal antibody (STJ117996) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using APAF1 rabbit polyclonal antibody (STJ117996) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using APAF1 rabbit polyclonal antibody (STJ117996) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-APAF1 antibody (470-600) (STJ117996)

SKU:
STJ117996

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APAF1
Gene ID: 317
Uniprot ID: APAF_HUMAN
Immunogen Region: 470-600
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 470-600 of human APAF1 (NP_863651.1).
Immunogen Sequence: PHTLSPDQEDCMYWYNFLAY HMASAKMHKELCALMFSLDW IKAKTELVGPAHLIHEFVEY RHILDEKDCAVSENFQEFLS LNGHLLGRQPFPNIVQLGLC EPETSEVYQQAKLQAKQEVD NGMLYLEWINK
Tissue Specificity Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
Function Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Protein Name Apoptotic Protease-Activating Factor 1
Apaf-1
Database Links Reactome: R-HSA-111458
Reactome: R-HSA-111459
Reactome: R-HSA-111463
Reactome: R-HSA-111464
Reactome: R-HSA-6798695
Reactome: R-HSA-6803207
Reactome: R-HSA-8953750
Reactome: R-HSA-9627069
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Apoptotic Protease-Activating Factor 1 antibody
Anti-Apaf-1 antibody
Anti-APAF1 antibody
Anti-KIAA0413 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance