• Immunofluorescence analysis of U-2 OS cells using AP3S2 Rabbit polyclonal antibody (STJ11102920) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using AP3S2 Rabbit polyclonal antibody (STJ11102920) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using AP3S2 Rabbit polyclonal antibody (STJ11102920) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-AP3S2 antibody (1-193) (STJ11102920)

SKU:
STJ11102920

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AP3S2 (1-193) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AP3S2
Gene ID: 10239
Uniprot ID: AP3S2_HUMAN
Immunogen Region: 1-193
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human AP3S2 (NP_005820.1).
Immunogen Sequence: MIQAILVFNNHGKPRLVRFY QRFPEEIQQQIVRETFHLVL KRDDNICNFLEGGSLIGGSD YKLIYRHYATLYFVFCVDSS ESELGILDLIQVFVETLDKC FENVCELDLIFHMDKVHYIL QEVVMGGMVLETNMNEIVAQ IEAQNRLEKSEGGLSAAPAR AVSAVKNINLPEIPRNINIG DLNIKVPNLSQFV
Tissue Specificity Present in all adult tissues examined.
Function Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.
Protein Name Ap-3 Complex Subunit Sigma-2
Ap-3 Complex Subunit Sigma-3b
Adaptor-Related Protein Complex 3 Subunit Sigma-2
Sigma-3b-Adaptin
Sigma3b-Adaptin
Sigma-Adaptin 3b
Cellular Localisation Golgi Apparatus
Cytoplasmic Vesicle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Component Of The Coat Surrounding The Cytoplasmic Face Of Coated Vesicles Located At The Golgi Complex
Alternative Antibody Names Anti-Ap-3 Complex Subunit Sigma-2 antibody
Anti-Ap-3 Complex Subunit Sigma-3b antibody
Anti-Adaptor-Related Protein Complex 3 Subunit Sigma-2 antibody
Anti-Sigma-3b-Adaptin antibody
Anti-Sigma3b-Adaptin antibody
Anti-Sigma-Adaptin 3b antibody
Anti-AP3S2 antibody

Information sourced from Uniprot.org

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