• Immunofluorescence analysis of Y79 cells using ANO2 Rabbit polyclonal antibody (STJ116212) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from mouse brain, using ANO2 Rabbit polyclonal antibody (STJ116212) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ANO2 antibody (1-200) (STJ116212)

SKU:
STJ116212

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ANO2 (1-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANO2
Gene ID: 57101
Uniprot ID: ANO2_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ANO2 (NP_001265526.1).
Immunogen Sequence: MPEDIPLLPGSPRRLSPQAG SRGGQGPKHGQQCLKMPGPR APGLQGGSNRDPGQPCGGES TRSSSVINNYLDANEPVSLE ARLSRMHFHDSQRKVDYVLA YHYRKRGVHLAQGFPGHSLA IVSNGETGKEPHAGGPGDIE LGPLDALEEERKEQREEFEH NLMEAGLELEKDLENKSQGS IFVRIHAPWQVLAREAEFLK
Tissue Specificity Retina, especially in the photoreceptor synaptic terminals.
Function Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina.
Protein Name Anoctamin-2
Transmembrane Protein 16b
Database Links Reactome: R-HSA-2672351
Reactome: R-HSA-381753
Reactome: R-HSA-9733458
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Anoctamin-2 antibody
Anti-Transmembrane Protein 16b antibody
Anti-ANO2 antibody
Anti-C12orf3 antibody
Anti-TMEM16B antibody

Information sourced from Uniprot.org

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