• Western blot analysis of extracts of various cell lines, using ANKRD28 antibody (STJ110663) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using ANKRD28 antibody (STJ110663) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ANKRD28 antibody (1-300) (STJ110663)

SKU:
STJ110663

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ANKRD28 (1-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANKRD28
Gene ID: 23243
Uniprot ID: ANR28_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ANKRD28 (NP_056014.2).
Immunogen Sequence: MAFLKLRDQPSLVQAIFNGD PDEVRALIFKKEDVNFQDNE KRTPLHAAAYLGDAEIIELL ILSGARVNAKDSKWLTPLHR AVASCSEEAVQVLLKHSADV NARDKNWQTPLHIAAANKAV KCAEALVPLLSNVNVSDRAG RTALHHAAFSGHGEMVKLLL SRGANINAFDKKDRRAIHWA AYMGHIEVVKLLVSHGAEVT CKDKKSYTPLHAAASSGMIS VVKYLLDLGVDMNEPNAYG
Function Putative regulatory subunit of protein phosphatase 6 (PP6) that may be involved in the recognition of phosphoprotein substrates. Involved in the PP6-mediated dephosphorylation of NFKBIE opposing its degradation in response to TNF-alpha. Selectively inhibits the phosphatase activity of PPP1C. Targets PPP1C to modulate HNRPK phosphorylation.
Protein Name Serine/Threonine-Protein Phosphatase 6 Regulatory Ankyrin Repeat Subunit A
Pp6-Ars-A
Serine/Threonine-Protein Phosphatase 6 Regulatory Subunit Ars-A
Ankyrin Repeat Domain-Containing Protein 28
Phosphatase Interactor Targeting Protein Hnrnp K
Pitk
Database Links Reactome: R-HSA-171319
Reactome: R-HSA-204005
Cellular Localisation Nucleus
Nucleoplasm
Seems To Be Excluded From Nucleoli
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase 6 Regulatory Ankyrin Repeat Subunit A antibody
Anti-Pp6-Ars-A antibody
Anti-Serine/Threonine-Protein Phosphatase 6 Regulatory Subunit Ars-A antibody
Anti-Ankyrin Repeat Domain-Containing Protein 28 antibody
Anti-Phosphatase Interactor Targeting Protein Hnrnp K antibody
Anti-Pitk antibody
Anti-ANKRD28 antibody
Anti-KIAA0379 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance