Anti-ANKRD28 antibody (1-300) (STJ110663)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ANKRD28 (1-300) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ANKRD28 |
| Gene ID | 23243 |
| Uniprot ID | ANR28_HUMAN |
| Immunogen Region | 1-300 |
| Immunogen Sequence | MAFLKLRDQPSLVQAIFNGD PDEVRALIFKKEDVNFQDNE KRTPLHAAAYLGDAEIIELL ILSGARVNAKDSKWLTPLHR AVASCSEEAVQVLLKHSADV NARDKNWQTPLHIAAANKAV KCAEALVPLLSNVNVSDRAG RTALHHAAFSGHGEMVKLLL SRGANINAFDKKDRRAIHWA AYMGHIEVVKLLVSHGAEVT CKDKKSYTPLHAAASSGMIS VVKYLLDLGVDMNEPNAYG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ANKRD28 (NP_056014.2). |
Additional Info
| Post Translational Modifications | Ubiquitinated by the ECS(RAB40C) complex leading to its degradation and decreased PP6 activity. |
| Function | Regulatory subunit of protein phosphatase 6 (PP6) that may be involved in the recognition of phosphoprotein substrates. Involved in the PP6-mediated dephosphorylation of NFKBIE opposing its degradation in response to TNF-alpha. Selectively inhibits the phosphatase activity of PPP1C. Targets PPP1C to modulate HNRPK phosphorylation. Involved in the PP6-mediated dephosphorylation of MOB1 and induced focal adhesion assembly during cell migration. |
| Protein Name | Serine/Threonine-Protein Phosphatase 6 Regulatory Ankyrin Repeat Subunit APp6-Ars-ASerine/Threonine-Protein Phosphatase 6 Regulatory Subunit Ars-AAnkyrin Repeat Domain-Containing Protein 28Phosphatase Interactor Targeting Protein Hnrnp KPitk |
| Database Links | Reactome: R-HSA-171319Reactome: R-HSA-204005 |
| Cellular Localisation | NucleusNucleoplasmCytoplasmCytosolCell ProjectionLamellipodiumSeems To Be Excluded From NucleoliMostly Localized In The CytosolBut A Fraction Could Be Observed At The Lamellipodia |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Phosphatase 6 Regulatory Ankyrin Repeat Subunit A antibodyAnti-Pp6-Ars-A antibodyAnti-Serine/Threonine-Protein Phosphatase 6 Regulatory Subunit Ars-A antibodyAnti-Ankyrin Repeat Domain-Containing Protein 28 antibodyAnti-Phosphatase Interactor Targeting Protein Hnrnp K antibodyAnti-Pitk antibodyAnti-ANKRD28 antibodyAnti-KIAA0379 antibody |
Information sourced from Uniprot.org