• Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100
  • Western blot analysis of 293T, Hela, 823 cells using ANG I Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ANG antibody (11-60 aa) (STJ97238)

SKU:
STJ97238

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Angiogenin (11-60 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANG
Gene ID: 283
Uniprot ID: ANGI_HUMAN
Immunogen Region: 11-60 aa
Specificity: ANG I Polyclonal Antibody detects endogenous levels of ANG I protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the N-terminal region of human N-ternal ANG at the amino acid range 11-60
Function Ribonuclease that cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Binds to actin on the surface of endothelial cells.once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
Protein Name Angiogenin
Ribonuclease 5
Rnase 5
Database Links Reactome: R-HSA-418990
Reactome: R-HSA-9708296
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle Lumen
Secreted
Nucleus
Nucleolus
Rapidly Endocytosed By Target Cells And Translocated To The Nucleus Where It Accumulates In The Nucleolus And Binds To Dna
Alternative Antibody Names Anti-Angiogenin antibody
Anti-Ribonuclease 5 antibody
Anti-Rnase 5 antibody
Anti-ANG antibody
Anti-RNASE5 antibody

Information sourced from Uniprot.org

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