• Immunofluorescence analysis of HeLa cells using Ambra1 antibody (STJ22601) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat lung using AMBRA1 antibody (STJ22601) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse lung using AMBRA1 antibody (STJ22601) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using Ambra1 Antibody (STJ22601) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.

Anti-Ambra1 antibody (STJ22601)

SKU:
STJ22601

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-Ambra1 antibody is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
Uniprot ID: AMRA1_HUMAN
Gene ID: 55626
Gene Symbol: AMBRA1
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1208 of human Ambra1 (NP_060219.2).
Protein Name Activating Molecule In Becn1-Regulated Autophagy Protein 1
Database Links Reactome: R-HSA-1632852
Cellular Localisation Cytoplasmic Vesicle
Autophagosome
Localizes Also To Discrete Punctae Along The Ciliary Axoneme
Alternative Antibody Names Anti-Activating Molecule In Becn1-Regulated Autophagy Protein 1 antibody
Anti-AMBRA1 antibody
Anti-KIAA1736 antibody

Information sourced from Uniprot.org

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