Confocal imaging ofย paraffin-embedded Rat heart usingย alpha Sarcoglycan (SGCA) Rabbit monoclonal antibody (STJ11103115, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-alpha Sarcoglycan antibody (288-387) [S5MR] (STJ11103115)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103115
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General Information

Short DescriptionRabbit monoclonal alpha Sarcoglycan (SGCA) (288-387) antibody for use in WB, IHC-P, IF, ICC and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityMouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS5MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:3000
IHC-P:1:200-1:800
IF/ICC:1:100-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSGCA
Gene ID6442
Uniprot IDSGCA_HUMAN
Immunogen Region288-387
Immunogen SequenceVDALVTLLVPLLVALLLTLL LAYVMCCRREGRLKRDLATS DIQMVHHCTIHGNTEELRQM AASREVPRPLSTLPMFNVHT GERLPPRVDSAQVPLILDQH
SpecificityA synthetic peptide corresponding to a sequence within amino acids 288-387 of human alpha Sarcoglycan (SGCA) (SGCA) (Q16586).

Additional Info

Tissue Specificity Most strongly expressed in skeletal muscle. Also expressed in cardiac muscle and, at much lower levels, in lung. In the fetus, most abundant in cardiac muscle and, at lower levels, in lung. Also detected in liver and kidney. Not expressed in brain.
Function Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Protein Name Alpha-Sarcoglycan
Alpha-Sg
50 Kda Dystrophin-Associated Glycoprotein
50dag
Adhalin
Dystroglycan-2
Database Links Reactome: R-HSA-9913351
Cellular Localisation Cell Membrane
Sarcolemma
Single-Pass Type I Membrane Protein
Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Alpha-Sarcoglycan antibody
Anti-Alpha-Sg antibody
Anti-50 Kda Dystrophin-Associated Glycoprotein antibody
Anti-50dag antibody
Anti-Adhalin antibody
Anti-Dystroglycan-2 antibody
Anti-SGCA antibody
Anti-ADL antibody
Anti-DAG2 antibody

Information sourced from Uniprot.org

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