• STJ70918 (0. 1µg/ml) staining of human liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ70918 (2. 5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-AKR1C4 antibody (N-Term) (STJ70918)

SKU:
STJ70918-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-AKR1C4 (N-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
IHC-Recommended concentration, 2-4µg/ml
ELISA-antibody detection limit dilution 1:4000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: AKR1C4
Gene ID: 1109
Uniprot ID: AK1C4_HUMAN
Immunogen Region: N-Term
Accession Number: NP_001809.2
Specificity: This antibody may cross-react with AKR1C1
Immunogen Sequence: DPKYQRVELNDGH
Post Translational Modifications The N-terminus is blocked.
Function Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Liver specific enzyme that acts as NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain. Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH. Acts preferentially as a 3-alpha-hydroxysteroid dehydrogenase (HSD) with a subsidiary 3-beta-HSD activity. Catalyzes efficiently the transformation of the potent androgen 5-alpha-dihydrotestosterone (5alpha-DHT or 17beta-hydroxy-5alpha-androstan-3-one) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). Catalyzes the reduction of estrone into 17beta-estradiol but with low efficiency. Metabolizes a broad spectrum of natural and synthetic therapeutic steroid and plays an important role in metabolism of androgens, estrogens, progestereone and conjugated steroids. Catalyzes the biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leading to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route.
Protein Name Aldo-Keto Reductase Family 1 Member C4
3-Alpha-Hydroxysteroid Dehydrogenase Type I
3-Alpha-Hsd1
3alpha-Hydroxysteroid 3-Dehydrogenase
Chlordecone Reductase
Cdr
Dihydrodiol Dehydrogenase 4
Dd-4
Dd4
Hakra
Database Links Reactome: R-HSA-193368
Reactome: R-HSA-193775
Reactome: R-HSA-193807
Reactome: R-HSA-975634
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Aldo-Keto Reductase Family 1 Member C4 antibody
Anti-3-Alpha-Hydroxysteroid Dehydrogenase Type I antibody
Anti-3-Alpha-Hsd1 antibody
Anti-3alpha-Hydroxysteroid 3-Dehydrogenase antibody
Anti-Chlordecone Reductase antibody
Anti-Cdr antibody
Anti-Dihydrodiol Dehydrogenase 4 antibody
Anti-Dd-4 antibody
Anti-Dd4 antibody
Anti-Hakra antibody
Anti-AKR1C4 antibody
Anti-CHDR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance