| Host: |
Rabbit |
| Applications: |
ELISA/IHC/IP/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-AKR1B1 (250-350) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
FITC |
| Isotype: |
IgG |
| Purification: |
Affinity Purified |
| Concentration: |
0.50 µg/µl |
| Dilution Range: |
WB: 1:500DB: 1:10, 000ELISA: 1:10, 000IP: 1:200IHC: 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
AKR1B1 |
| Gene ID: |
231 |
| Uniprot ID: |
ALDR_HUMAN |
| Immunogen Region: |
250-350 |
| Immunogen: |
Synthetic peptide taken within amino acid region 250-350 on human Aldo-keto reductase family 1, member B1 protein. |
| Tissue Specificity | Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress. |
| Function | Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia. Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides. Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls). |
| Protein Name | Aldo-Keto Reductase Family 1 Member B1 Aldehyde ReductaseAldose ReductaseAr |
| Database Links | Reactome: R-HSA-196108Reactome: R-HSA-5652227 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Aldo-Keto Reductase Family 1 Member B1 antibodyAnti-Aldehyde Reductase antibodyAnti-Aldose Reductase antibodyAnti-Ar antibodyAnti-AKR1B1 antibodyAnti-ALDR1 antibodyAnti-ALR2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and AKR1B1 knockout (KO) HeLa cells, using [KO Validated] AKR1B1 Rabbit polyclonal antibody (STJ11100004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89077/357534/STJ11100004_1__87524.1713121649.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AKR1B1 knockout (KO) HeLa cells, using [KO Validated] AKR1B1 Rabbit polyclonal antibody (STJ11100004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
