• Immunofluorescence analysis of rat testis cells using AKAP4 Rabbit polyclonal antibody (STJ117013) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of human testis cells using AKAP4 Rabbit polyclonal antibody (STJ117013) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using AKAP4 antibody (STJ117013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AKAP4 antibody (189-340) (STJ117013)

SKU:
STJ117013

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AKAP4 (189-340) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKAP4
Gene ID: 8852
Uniprot ID: AKAP4_HUMAN
Immunogen Region: 189-340
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 189-340 of human AKAP4 (NP_003877.2).
Immunogen Sequence: QSPSAPPAKPPSTQRAVISP DGECSIDDLSFYVNRLSSLV IQMAHKEIKEKLEGKSKCLH HSICPSPGNKERISPRTPAS KIASEMAYEAVELTAAEMRG TGEESREGGQKSFLYSELSN KSKSGDKQMSQRESKEFADS ISKGLMVYANQV
Tissue Specificity Testis specific.only expressed in round spermatids.
Function Major structural component of sperm fibrous sheath. Plays a role in sperm motility.
Protein Name A-Kinase Anchor Protein 4
Akap-4
A-Kinase Anchor Protein 82 Kda
Akap 82
Hakap82
Major Sperm Fibrous Sheath Protein
Hi
Protein Kinase A-Anchoring Protein 4
Prka4
Cellular Localisation Cell Projection
Cilium
Flagellum
Localizes To The Principle Piece Of The Sperm Flagellum
Alternative Antibody Names Anti-A-Kinase Anchor Protein 4 antibody
Anti-Akap-4 antibody
Anti-A-Kinase Anchor Protein 82 Kda antibody
Anti-Akap 82 antibody
Anti-Hakap82 antibody
Anti-Major Sperm Fibrous Sheath Protein antibody
Anti-Hi antibody
Anti-Protein Kinase A-Anchoring Protein 4 antibody
Anti-Prka4 antibody
Anti-AKAP4 antibody
Anti-AKAP82 antibody

Information sourced from Uniprot.org

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