Anti-AK4 antibody (1-223) (STJ115422)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal AK4 (1-223) antibody for use in WB, IP and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AK4 |
| Gene ID | 205 |
| Uniprot ID | KAD4_HUMAN |
| Immunogen Region | 1-223 |
| Immunogen Sequence | MASKLLRAVILGPPGSGKGT VCQRIAQNFGLQHLSSGHFL RENIKASTEVGEMAKQYIEK SLLVPDHVITRLMMSELENR RGQHWLLDGFPRTLGQAEAL DKICEVDLVISLNIPFETLK DRLSRRWIHPPSGRVYNLDF NPPHVHGIDDVTGEPLVQQE DDKPEAVAARLRQYKDVAKP VIELYKSRGVLHQFSGTETN KIWPYVYTLFSNKITPIQSK EAY |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human AK4 (NP_037542.1). |
Additional Info
| Tissue Specificity | Highly expressed in kidney, moderately expressed in heart and liver and weakly expressed in brain. |
| Function | Broad-specificity mitochondrial nucleoside phosphate kinase involved in cellular nucleotide homeostasis by catalyzing nucleoside-phosphate interconversions. Similar to other adenylate kinases, preferentially catalyzes the phosphorylation of the nucleoside monophosphate AMP with ATP as phosphate donor to produce ADP. Phosphorylates only AMP when using GTP as phosphate donor. In vitro, can also catalyze the phosphorylation of CMP, dAMP and dCMP and use GTP as an alternate phosphate donor. Moreover, exhibits a diphosphate kinase activity, producing ATP, CTP, GTP, UTP, TTP, dATP, dCTP and dGTP from the corresponding diphosphate substrates with either ATP or GTP as phosphate donors. Plays a role in controlling cellular ATP levels by regulating phosphorylation and activation of the energy sensor protein kinase AMPK. Plays a protective role in the cellular response to oxidative stress. |
| Protein Name | Adenylate Kinase 4 - MitochondrialAdenylate Kinase 3-LikeGtp -Amp Phosphotransferase Ak4 |
| Database Links | Reactome: R-HSA-499943 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Adenylate Kinase 4 - Mitochondrial antibodyAnti-Adenylate Kinase 3-Like antibodyAnti-Gtp -Amp Phosphotransferase Ak4 antibodyAnti-AK4 antibodyAnti-AK3 antibodyAnti-AK3L1 antibody |
Information sourced from Uniprot.org