| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-AIRE (1-280) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
AIRE |
| Gene ID: |
326 |
| Uniprot ID: |
AIRE_HUMAN |
| Immunogen Region: |
1-280 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human AIRE (NP_000374.1). |
| Immunogen Sequence: |
MATDAALRRLLRLHRTEIAV AVDSAFPLLHALADHDVVPE DKFQETLHLKEKEGCPQAFH ALLSWLLTQDSTAILDFWRV LFKDYNLERYGRLQPILDSF PKDVDLSQPRKGRKPPAVPK ALVPPPRLPTKRKASEEARA AAPAALTPRGTASPGSQLKA KPPKKPESSAEQQRLPLGNG IQTMSASVQRAVAMSSGDVP GARGAVEGILIQQVFESGGS KKCIQVGGEFYTPSKFEDS |
| Tissue Specificity | Widely expressed. Expressed at higher level in thymus (medullary epithelial cells and monocyte-dendritic cells), pancreas, adrenal cortex and testis. Expressed at lower level in the spleen, fetal liver and lymph nodes. In secondary lymphoid organs, expressed in a discrete population of bone marrow-derived toleregenic antigen presenting cells (APCs) called extrathymic AIRE expressing cells (eTAC)(at protein level). Isoform 2 and isoform 3 seem to be less frequently expressed than isoform 1, if at all. |
| Post Translational Modifications | Phosphorylated. Phosphorylation could trigger oligomerization. |
| Function | Transcription factor playing an essential role to promote self-tolerance in the thymus by regulating the expression of a wide array of self-antigens that have the commonality of being tissue-restricted in their expression pattern in the periphery, called tissue restricted antigens (TRA). Binds to G-doublets in an A/T-rich environment.the preferred motif is a tandem repeat of 5'-ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes. Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Mainly expressed by medullary thymic epithelial cells (mTECs), induces the expression of thousands of tissue-restricted proteins, which are presented on major histocompatibility complex class I (MHC-I) and MHC-II molecules to developing T-cells percolating through the thymic medulla. Also induces self-tolerance through other mechanisms such as the regulation of the mTEC differentiation program. Controls the medullary accumulation of thymic dendritic cells and the development of regulatory T-cell through the regulation of XCL1 expression. Regulates the production of CCR4 and CCR7 ligands in medullary thymic epithelial cells and alters the coordinated maturation and migration of thymocytes. In thimic B-cells, allows the presentation of licensing-dependent endogenous self-anitgen for negative selection. In secondary lymphoid organs, induces functional inactivation of CD4(+) T-cells. Expressed by a distinct bone marrow-derived population, induces self-tolerance through a mechanism that does not require regulatory T-cells and is resitant to innate inflammatory stimuli. |
| Protein Name | Autoimmune RegulatorAutoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy ProteinApeced Protein |
| Cellular Localisation | NucleusCytoplasmPredominantly Nuclear But Also CytoplasmicFound In Nuclear Body-Like Structures (Dots) And In A Filamentous Vimentin-Like PatternAssociated With Tubular Structures |
| Alternative Antibody Names | Anti-Autoimmune Regulator antibodyAnti-Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy Protein antibodyAnti-Apeced Protein antibodyAnti-AIRE antibodyAnti-APECED antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
