• Western blot analysis of extracts of various cell lines, using AIRE antibody (STJ116116) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AIRE antibody (1-280) (STJ116116)

SKU:
STJ116116

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AIRE (1-280) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AIRE
Gene ID: 326
Uniprot ID: AIRE_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human AIRE (NP_000374.1).
Immunogen Sequence: MATDAALRRLLRLHRTEIAV AVDSAFPLLHALADHDVVPE DKFQETLHLKEKEGCPQAFH ALLSWLLTQDSTAILDFWRV LFKDYNLERYGRLQPILDSF PKDVDLSQPRKGRKPPAVPK ALVPPPRLPTKRKASEEARA AAPAALTPRGTASPGSQLKA KPPKKPESSAEQQRLPLGNG IQTMSASVQRAVAMSSGDVP GARGAVEGILIQQVFESGGS KKCIQVGGEFYTPSKFEDS
Tissue Specificity Widely expressed. Expressed at higher level in thymus (medullary epithelial cells and monocyte-dendritic cells), pancreas, adrenal cortex and testis. Expressed at lower level in the spleen, fetal liver and lymph nodes. In secondary lymphoid organs, expressed in a discrete population of bone marrow-derived toleregenic antigen presenting cells (APCs) called extrathymic AIRE expressing cells (eTAC)(at protein level). Isoform 2 and isoform 3 seem to be less frequently expressed than isoform 1, if at all.
Post Translational Modifications Phosphorylated. Phosphorylation could trigger oligomerization.
Function Transcription factor playing an essential role to promote self-tolerance in the thymus by regulating the expression of a wide array of self-antigens that have the commonality of being tissue-restricted in their expression pattern in the periphery, called tissue restricted antigens (TRA). Binds to G-doublets in an A/T-rich environment.the preferred motif is a tandem repeat of 5'-ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes. Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Mainly expressed by medullary thymic epithelial cells (mTECs), induces the expression of thousands of tissue-restricted proteins, which are presented on major histocompatibility complex class I (MHC-I) and MHC-II molecules to developing T-cells percolating through the thymic medulla. Also induces self-tolerance through other mechanisms such as the regulation of the mTEC differentiation program. Controls the medullary accumulation of thymic dendritic cells and the development of regulatory T-cell through the regulation of XCL1 expression. Regulates the production of CCR4 and CCR7 ligands in medullary thymic epithelial cells and alters the coordinated maturation and migration of thymocytes. In thimic B-cells, allows the presentation of licensing-dependent endogenous self-anitgen for negative selection. In secondary lymphoid organs, induces functional inactivation of CD4(+) T-cells. Expressed by a distinct bone marrow-derived population, induces self-tolerance through a mechanism that does not require regulatory T-cells and is resitant to innate inflammatory stimuli.
Protein Name Autoimmune Regulator
Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy Protein
Apeced Protein
Cellular Localisation Nucleus
Cytoplasm
Predominantly Nuclear But Also Cytoplasmic
Found In Nuclear Body-Like Structures (Dots) And In A Filamentous Vimentin-Like Pattern
Associated With Tubular Structures
Alternative Antibody Names Anti-Autoimmune Regulator antibody
Anti-Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy Protein antibody
Anti-Apeced Protein antibody
Anti-AIRE antibody
Anti-APECED antibody

Information sourced from Uniprot.org

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