• Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from COS7 cells, using AIFM2 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using AMID Polyclonal Antibody

Anti-AIFM2 antibody (141-190 aa) (STJ91575)

SKU:
STJ91575

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ferroptosis suppressor protein 1 (141-190 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AIFM2
Gene ID: 84883
Uniprot ID: FSP1_HUMAN
Immunogen Region: 141-190 aa
Specificity: AMID Polyclonal Antibody detects endogenous levels of AMID protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human AIFM2 at the amino acid range 141-190
Post Translational Modifications N-myristoylation at Gly-2 mediates the recruitment to lipid droplets and plasma membrane.
Function A NAD(P)H-dependent oxidoreductase that acts as a key inhibitor of ferroptosis. At the plasma membrane, catalyzes reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a lipophilic radical-trapping antioxidant that prevents lipid oxidative damage and consequently ferroptosis. Acts in parallel to GPX4 to suppress phospholipid peroxidation and ferroptosis. This anti-ferroptotic function is independent of cellular glutathione levels. Also acts as a potent radical-trapping antioxidant by mediating warfarin-resistant vitamin K reduction in the canonical vitamin K cycle: catalyzes NAD(P)H-dependent reduction of vitamin K (phylloquinone, menaquinone-4 and menadione) to hydroquinone forms. Hydroquinones act as potent radical-trapping antioxidants inhibitor of phospholipid peroxidation and ferroptosis. May play a role in mitochondrial stress signaling. Upon oxidative stress, associates with the lipid peroxidation end product 4-hydroxy-2-nonenal (HNE) forming a lipid adduct devoid of oxidoreductase activity, which then translocates from mitochondria into the nucleus triggering DNA damage and cell death. Capable of DNA binding in a non-sequence specific way.
Protein Name Ferroptosis Suppressor Protein 1
Fsp1
Apoptosis-Inducing Factor Homologous Mitochondrion-Associated Inducer Of Death
Amid
P53-Responsive Gene 3 Protein
Database Links Reactome: R-HSA-6803204
Cellular Localisation Lipid Droplet
Cell Membrane
Lipid-Anchor
Cytoplasm
Mitochondrion Membrane
Nucleus
Alternative Antibody Names Anti-Ferroptosis Suppressor Protein 1 antibody
Anti-Fsp1 antibody
Anti-Apoptosis-Inducing Factor Homologous Mitochondrion-Associated Inducer Of Death antibody
Anti-Amid antibody
Anti-P53-Responsive Gene 3 Protein antibody
Anti-AIFM2 antibody
Anti-AMID antibody
Anti-PRG3 antibody

Information sourced from Uniprot.org

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