• Western blot analysis of extracts of various cell lines, using AGTRAP antibody (STJ114071) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of U2OS cells using AGTRAP rabbit polyclonal antibody (STJ114071) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AGTRAP antibody (93-152) (STJ114071)

SKU:
STJ114071

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AGTRAP (93-152) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AGTRAP
Gene ID: 57085
Uniprot ID: ATRAP_HUMAN
Immunogen Region: 93-152
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 93-152 of human AGTRAP (NP_065083.3).
Immunogen Sequence: ILSLLLKPLSCCFVYHMYRE RGGELLVHTGFLGSSQDRSA YQTIDSAEAPADPFAVPEGR
Tissue Specificity Ubiquitous but more abundant in kidney, heart, pancreas and thyroid.
Function Appears to be a negative regulator of type-1 angiotensin II receptor-mediated signaling by regulating receptor internalization as well as mechanism of receptor desensitization such as phosphorylation. Induces also a decrease in cell proliferation and angiotensin II-stimulated transcriptional activity.
Protein Name Type-1 Angiotensin Ii Receptor-Associated Protein
At1 Receptor-Associated Protein
Database Links Reactome: R-HSA-6802952
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Golgi Apparatus Membrane
Cytoplasmic Vesicle Membrane
Present In Perinuclear Vesicular Membranes
Endoplasmic Reticulum
Golgi And Endocytic Vesicles
Alternative Antibody Names Anti-Type-1 Angiotensin Ii Receptor-Associated Protein antibody
Anti-At1 Receptor-Associated Protein antibody
Anti-AGTRAP antibody
Anti-ATRAP antibody

Information sourced from Uniprot.org

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