• Western blot analysis of extracts of various cell lines, using Argonaute-2 antibody (STJ11101778) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Argonaute-2 antibody (STJ11101778) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Argonaute-2 in paraffin-embedded human colon carcinoma tissue using Argonaute-2 rabbit monoclonal antibody (STJ11101778) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Argonaute-2 in paraffin-embedded mouse liver tissue using Argonaute-2 rabbit monoclonal antibody (STJ11101778) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Argonaute-2 in paraffin-embedded rat kidney tissue using Argonaute-2 rabbit monoclonal antibody (STJ11101778) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Argonaute-2 in paraffin-embedded rat liver tissue using Argonaute-2 rabbit monoclonal antibody (STJ11101778) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-AGO2 antibody (350-450) [S8MR] (STJ11101778)

SKU:
STJ11101778

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Argonaute-2 (350-450) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AGO2
Gene ID: 27161
Uniprot ID: AGO2_HUMAN
Immunogen Region: 350-450
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Argonaute-2 (Q9UKV8).
Immunogen Sequence: QRCIKKLTDNQTSTMIRATA RSAPDRQEEISKLMRSASFN TDPYVREFGIMVKDEMTDVT GRVLQPPSILYGGRNKAIAT PVQGVWDMRNKQFHTGIEIK V
Post Translational Modifications Hydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity. Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 during target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). Ubiquitination by the SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 leads to its subsequent degradation, thereby exposing miRNAs for degradation. ZSWIM8 recognizes and binds AGO2 when it is engaged with a TDMD target. Phosphorylated. A phosphorylation cycle of C-terminal serine cluster (Ser-824-Ser-834) regulates the release of target mRNAs. Target-binding leads to phosphorylation of these residues by CSNK1A1, which reduces the affinity of AGO2 for mRNA and enables target release. The ANKRD52-PPP6C phosphatase complex dephosphorylates the residues, which primes AGO2 for binding a new target. Phosphorylation at Ser-387 by AKT3.leads to up-regulate translational repression of microRNA target and down-regulate endonucleolytic cleavage.
Function Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. (Microbial infection) Upon Sars-CoV-2 infection, associates with viral miRNA-like small RNA, CoV2-miR-O7a, and may repress mRNAs, such as BATF2, to evade the IFN response.
Protein Name Protein Argonaute-2
Argonaute2
Hago2
Argonaute Risc Catalytic Component 2
Eukaryotic Translation Initiation Factor 2c 2
Eif-2c 2
Eif2c 2
Paz Piwi Domain Protein
Ppd
Protein Slicer
Database Links Reactome: R-HSA-1912408
Reactome: R-HSA-203927
Reactome: R-HSA-4086398
Reactome: R-HSA-426486
Reactome: R-HSA-426496
Reactome: R-HSA-5578749
Reactome: R-HSA-5628897
Reactome: R-HSA-5687128
Reactome: R-HSA-8934593
Reactome: R-HSA-8943723
Reactome: R-HSA-8948700
Reactome: R-HSA-8986944
Reactome: R-HSA-9018519
Reactome: R-HSA-9022692
Reactome: R-HSA-9029569
Reactome: R-HSA-9759811
Reactome: R-HSA-9768778
Cellular Localisation Cytoplasm
P-Body
Nucleus
Translational Repression Of Mrnas Results In Their Recruitment To P-Bodies
Translocation To The Nucleus Requires Imp8
Alternative Antibody Names Anti-Protein Argonaute-2 antibody
Anti-Argonaute2 antibody
Anti-Hago2 antibody
Anti-Argonaute Risc Catalytic Component 2 antibody
Anti-Eukaryotic Translation Initiation Factor 2c 2 antibody
Anti-Eif-2c 2 antibody
Anti-Eif2c 2 antibody
Anti-Paz Piwi Domain Protein antibody
Anti-Ppd antibody
Anti-Protein Slicer antibody
Anti-AGO2 antibody
Anti-EIF2C2 antibody

Information sourced from Uniprot.org

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