• Western blot analysis of extracts of various cell lines, using AGA antibody (STJ115877) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AGA antibody (24-346) (STJ115877)

SKU:
STJ115877

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AGA (24-346) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AGA
Gene ID: 175
Uniprot ID: ASPG_HUMAN
Immunogen Region: 24-346
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-346 of human AGA (NP_000018.2).
Immunogen Sequence: SSPLPLVVNTWPFKNATEAA WRALASGGSALDAVESGCAM CEREQCDGSVGFGGSPDELG ETTLDAMIMDGTTMDVGAVG DLRRIKNAIGVARKVLEHTT HTLLVGESATTFAQSMGFIN EDLSTTASQALHSDWLARNC QPNYWRNVIPDPSKYCGPYK PPGILKQDIPIHKETEDDRG HDTIGMVVIHKTGHIAAGTS TNGIKFKIHGRVGDSPIPGA GAYADDTAGAAAATGNGDI
Post Translational Modifications Cleaved into an alpha and beta chain by autocatalysis.this activates the enzyme. The N-terminal residue of the beta subunit is responsible for the nucleophile hydrolase activity. N-glycosylated.
Function Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins.
Protein Name N(4-(Beta-N-Acetylglucosaminyl-L-Asparaginase
Aspartylglucosaminidase
Glycosylasparaginase
N4-(N-Acetyl-Beta-Glucosaminyl-L-Asparagine Amidase Cleaved Into - Glycosylasparaginase Alpha Chain - Glycosylasparaginase Beta Chain
Database Links Reactome: R-HSA-6798695
Cellular Localisation Lysosome
Alternative Antibody Names Anti-N(4-(Beta-N-Acetylglucosaminyl-L-Asparaginase antibody
Anti-Aspartylglucosaminidase antibody
Anti-Glycosylasparaginase antibody
Anti-N4-(N-Acetyl-Beta-Glucosaminyl-L-Asparagine Amidase Cleaved Into - Glycosylasparaginase Alpha Chain - Glycosylasparaginase Beta Chain antibody
Anti-AGA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance