• Immunofluorescence analysis of paraffin-embedded human liver cancer using Alpha-Fetoprotein (AFP) Mouse monoclonal antibody (STJ113435) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Alpha-Fetoprotein (AFP) in paraffin-embedded human liver cancer using Alpha-Fetoprotein (AFP) Mouse monoclonal antibody (STJ113435) at dilution of 1:12. 5 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HepG2 cells, using Alpha-Fetoprotein (AFP) Mouse monoclonal antibody (STJ113435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AFP antibody [S5MM] (STJ113435)

SKU:
STJ113435

£212.50 - £501.50
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Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-AFP is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MM
Conjugation: Unconjugated
Isotype: IgG2bk
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AFP
Gene ID: 174
Uniprot ID: FETA_HUMAN
Immunogen: Recombinant protein of human Alpha-Fetoprotein (Alpha-Fetoprotein (AFP) )
Immunogen Sequence: Email for sequence
Tissue Specificity Plasma. Synthesized by the fetal liver and yolk sac.
Post Translational Modifications Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated.
Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Protein Name Alpha-Fetoprotein
Alpha-1-Fetoprotein
Alpha-Fetoglobulin
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative Antibody Names Anti-Alpha-Fetoprotein antibody
Anti-Alpha-1-Fetoprotein antibody
Anti-Alpha-Fetoglobulin antibody
Anti-AFP antibody
Anti-HPAFP antibody

Information sourced from Uniprot.org

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